Manolis Kellis
@manoliskellis1·24.9K subscribers·255 videos
MIT CompBio Lecture 24 - Genome Engineering (Fall 2019)
Posted
December 5, 2019
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1,537
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December 5, 2019
1,537
23
3
1.69%
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1:18:40Now PlayingMIT Computational Biology: Genomes, Networks, Evolution, Health
Prof. Manolis Kellis
Full playlist with all videos in order is here
All slides from Fall 2019 are here
Outline for this lecture:
1. High-throughput synthesis: Massively Parallel Reporter Assays (MPRA)
- MPRA technology: in vitro synthesis, reporter design, transfection
- 1 site X deep: single-base dissection, combinatorial changes, high-res map
- 2k sites x shallow: motif-guided perturbations, activators/repressors
2. Next-generation applications of MPRA + related technologies
- 10k x deep: high-resolution dissection by tiling + deconvolution (Sharpr)
- 10M x deep: HiDRA, no synthesis (STARR), targeted capture (ATAC)
3. Endogenous genome editing: cutting and template-based repair
- Endonucleases: TALENs, ZNFs. Repair pathways. Diversity of outcomes.
- CRISPR-Cas9: origins, discovery, optimization, base-editing
- Next-generation: multiplexing, delivery, applications
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