Manolis Kellis
@manoliskellis1·24.9K subscribers·255 videos
MIT CompBio Lecture 09 - Three Dimensional Genome
Posted
October 5, 2018
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October 5, 2018
7,440
173
5
2.39%
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1:18:47Now PlayingMIT Computational Biology: Genomes, Networks, Evolution, Health
Prof. Manolis Kellis
Fall 2018
Lecture 09 - Three Dimensional Genome
1. Methods for studying nuclear genome organization
- Measuring locus-landmark interactions: ChIP, DamID
- Measuring locus-locus interactions: 3C, 4C, 5C, Hi-C, ChIA-PET
2. Processing and normalization of Hi-C datasets
- Correcting for biases and issues with Hi-C data
- Normalization, sources of bias and bias correction
3. Interpretation of data and functional characterization
- Lamina Associated Domains (LADs)
- Topologically Associated Domains (TADs) and other compartments
4. Conservation of nuclear genome organization
- Across species, across cell types
- Sequence basis for conservation?
5. Towards a mechanistic understanding
- Nuclear genome organization through the (single) cell cycle
- (Limitations of) 3D modeling of nuclear genome organization
- Loop extrusion as a potential mechanism for nuclear organization
Slides for Lecture 9:
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